Introduction RNA interference

Introduction
RNA interference (RNAi) also known as Post-Transcriptional Gene Silencing (PTGS)…. is extensively used as a research tool that offer particular approaches for the silencing or down-regulation of particular gene expression, through the degradation of specific messenger RNAs (mRNAs)…..
Mechanism:
The principle of RNA interference (RNAi) is downregulation of target gene expression for study of biological systems and implementation of an artificial genetic circuits that function by altering RNA concentrations ….. RNA interference make use of small interfering RNAs of 20–25 nucleotides processed from double-stranded RNA to initiate sequence-specific breakdown of the related messenger RNA. Ribonucleic acid interference (RNAi) shows potential for gene knockdown by inhibiting the expression of target genes in a sequence-specific manner, and promises to revolutionize experimental biology and may have significant applications in agriculture, functional genomics, enhancement of productivity, therapeutic interventions and other areas….The high degrees of specificity and efficiency are the main advantages of RNAi. Activation of RNAi pathway can be exogenous or endogenous double-stranded RNAs (dsRNAs). Endogenous triggers of RNAi pathway involve double-stranded RNA (dsRNA) or foreign DNA of viral origin, abnormal transcripts from repetitive sequences in the genome such as pre-microRNA (miRNA), and transposons. In plants, RNAi forms the basis of virus-induced gene silencing (VIGS), signifying its role in pathogenic resistance.

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